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Bioactivity-Guided Fractionation of Aqueous Leaf Extract Oftephrosia Vogelii And its Effect on Hyperlipidemia in Rats

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– Bioactivity-Guided Fractionation of Aqueous Leaf Extract Oftephrosia Vogelii And its Effect on Hyperlipidemia in Rats –

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Abstract

There are claims by traditional medicine practitioners in Nigeria that the leaves of Tephrosia vogeliihook. f. are used for the treatment of cardiovascular diseases but it appears there is no scientific research and publication or documented work to verify these claims.

This study aimed for bioactivity guided fractionation of aqueous leaves extract ofT. vogeliiand its effect on hyperlipidemic rats.

The induction of hyperlipidemia was carried out with high fat diet and Poloxamer 407 (P-407). Partial purification and identification were carried out using Thin Layer Chromatography (TLC), Fourier Transform Infrared Spectroscopy (FTIR) and Gas Chromatography- Mass Spectra (GC-MS).

Introduction

Cardiovascular diseases (CVDs) such asmyocardial infarction (heart attacks), coronary heart disease, high blood pressure, peripheral vascular diseases and stroke are the leading causes of death for both men and women among all racial and ethnic groups (Smith, 2004).

They lead to nearly 50% of all deaths in the developed world (Thomas and Rich, 2007; Mendis et al., 2011).

If this continues, the future will even be more challenging considering that globalization and wide spread of western diet to the developing world resulting to high rates of obesity and hyperlipidemia in developing countries (Lim et al., 2012).

Thus, hyperlipidemia and its associated CVDs are considered as one of the highest worldwide economic, social and medical challenges (Olshanky et al., 2005).

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