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Comparative Antibacterial, Anti-Venom And Phytochemical Studies Of Indigofera Capitata Kotschy And Indigofera Conferta Gillett In Albino Rats.

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– Comparative Antibacterial, Anti-Venom And Phytochemical Studies Of Indigofera Capitata Kotschy And Indigofera Conferta Gillett In Albino Rats –

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Abstract

This study was conducted to establish and compare anti-bacterial, anti-venom andphytochemical properties of Indigofera capitata and I. conferta in albino rats.

The twospecies of plants belong to the family Fabaceae. They are found growing on red soil orold farm land. The two plant materials were collected, dried, grounded and successivelyextracted using maceration with petroleum ether, ethanol and aqueous.

The extract ofeach solvent of the two plants was used for acute toxicity studies, in-vitro anti-bacterialand in-vivo anti-venom studies: Oedema forming activity, bleeding and clotting timeand histopathological examination of liver, kidney and heart were also carried out.

The most active extracts (ethanol and aqueous) were further subjected to thin layerchromatographic (TLC) studies. Antibacterial activities of all the extracts (petroleumether, ethanol and aqueous) of both plants showed significant effects (P<0.05) againstthe growth of Staphylococcus aureus and Salmonella typhi.

Indigofera confertapetroleum ether extract had MIC of 6.2 mg/ml and 12.5 mg/ml and MBC of 12.5 mg/mland 25.0 mg/ml, ethanol extract showed MIC of 12.5 mg/ml and MBC of 25.0 mg/mland aqueous extract showed MIC of 6.25

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