Monostaining Of Kidney And Liver Tissues With Extracts Of Tumeric (Curcuma Longa) And Zobo (Hibiscus Sabdariffa)

Description

– Monostaining Of Kidney And Liver Tissues With Extracts Of Tumeric (Curcuma Longa) And Zobo (Hibiscus Sabdariffa) –

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Abstract

Monstaining is a special kind of staining procedure that highlights single cells.

Most stains used in histopathology are synthetic, toxic and costly for use hence this study was carried out at the histopathology laboratory of Imo state university to research more on the probability of replacing routine toxic and costly stains for natural,

non toxic, less costly and effective stains that can highlight observably single cellular structures when viewed microscopically.

Twelve sections were used for this study (6 liver and 6 kidney sections), obtained from the federal medical center Owerri of which was divided into four groups of liver and kidney sections respectively.

Introduction

A stain is a dye consisting of a colored ion (a chromophore) and a counter ion to balance the charge. Attachment of the chromophore part of the dye complex to a cellular component represents the staining reaction.

Monostaining or Monochrome staining is the use of a single colour stain to colour tissues. It is mainly used on bacterial cells (Gurr, 2010).

Simple staining implies the use of only a single stain, which is usually sufficient to reveal the morphological features of most microbial cells, including relative size, shape, and characteristic arrangements for groups of cells.

It is possible to see structural details by staining with a single dye if the sections are sufficiently thin. To obtain additional information, various specialized differential staining procedures have been developed.

Examples of monostains used includes Heidenhain’s iron-hematoxylin which is a classical method of this type,  Eriochrome cyanine R an anionic hydroxytriarylmethane dye that has various uses as an industrial colorant, a reagent in analytical chemistry and a biological stain.

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