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The Potential of Cysteine Protease-encoding-gene as Candidate for DNA Vaccine against Plasmodium Berghei Infection in Mice

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– The Potential of Cysteine Protease-encoding-gene as Candidate for DNA Vaccine against Plasmodium Berghei Infection in Mice –

Download The Potential of Cysteine Protease-encoding-gene as Candidate for DNA Vaccine against Plasmodium Berghei Infection in Mice. Biochemistry students who are writing their projects can get this material to aid their research work.

Abstract

The potential of cysteine protease-DNA -vaccine- constructs to protect mice against Plasmodium berghei infection was investigated.

The full-length genes encoding the orthologs of falcipain-1 and falcipain-2 were identified in the database, isolated and amplified by a nested polymerase chain reaction (PCR) using the genomic DNA of a rodent malaria parasite, Plasmodium berghei, as template.

The oligonucleotides used to prime the PCR were designed based on the identified contigs at the Welcome Trust Sanger Institute containing the entire cysteine proteases reading frames.

The gel purified amplicons were cloned into pcDNA3AmpStrepTag vector and the presence of the genes in positively transformed XL1-Blue E. coli strain were confirmed via colony PCR, restriction digest and sequencing.

Introduction

Malaria is a life-threatening parasitic disease caused by various species of plasmodia and is transmitted via the bite of mosquitoes. It is thought to be man’s most important parasitic disease.

According to the World Health Organization there are 300 to 500 million clinical cases of malaria each year resulting in 1.5 to 2.7 million deaths (IDR centre report, 1996) with SubSaharan Africa known to be the region with the highest malaria infection rate.

In fact, it is reported that 80 -90 % of malarial deaths occur in this region (IDR centre report, 1996). Four plasmodium species (Plasmodium falciparum, Plasmodium vivax,

Plasmodium malariae and Plasmodium ovale) are responsible for malaria infection among human with P. falciparum infection accounting for most of the death cases encountered (Orjih, 2005) that is why research efforts on malaria has focused largely on P. falciparum.

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