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Total Alkaloids, Total Tannins Content and Antiulcer Assay of Four Selected Medicinal Plants

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Description

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Abstract

The total alkaloid, tannins contents and antiulcer activity of the extracts from four selected medicinal plants were investigated. The total alkaloids content (TAC) was evaluated according to the chloride colometric method in which atropine was used as standard.

While the total tannins content (TTC) was also determined using Folin Ciocalteau assay in which gallic acid was used as a standard.

The antiulcer activity of the extracts was investigated using the ethanol induced model in wistar albino rats. Parameters such as gastric volume, pH and ulcer index were used as indicators for the antiulcerogenic activity of the extracts.

The animals were orally treated with distilled water (Normal control group), ranitidine 5 mg/kg (standard control group), 0.5 mg/kg of ethanol (Negative control group) and 100,200 and 400 mg/kg of the extracts, 1 h before oral administration of absolute ethanol to induce gastric mucosal injury.

The result showed that E. deightonii extract has the richest source of alkaloids and tannins (0.850 ± 0.001 mg AE/g and 0.133 ± 0.001 mg GAE/g respectively, while A. hispidum and P. staudtii has the least TAC and TTC (0.800 ± 0.001 mg AE/g) and (0.124 ± 0.001 mg GAE/g) respectively.

Introduction

Plants are gifts from God and have been utilized by human beings for basic preventive and curative healthcare since time immemorial1.

In recent years, following the demonstration of the presence of active principles against many diseases and infections in a variety of plants extracts, there have been a great surge of public interest in the use of herbs and plants.

This phenomenon has been viewed by some scientists as modern Herbal- renaissance2. Though, pharmaceutical industries are moving towards synthetics and biotechnology research in search of novel medicine in the 21st century, consumers are expressing a greater awareness of the risk of using synthetic products3.

Therefore, attention is now focused on the various medicinal plants, their potentials, safety and their levels of toxicities if any 4.

According to the World Health Organization (WHO), a medicinal plant is any plant which, in one or more of its organs contain substances that can be used for therapeutic purposes or which are precursors for the syntheses of useful drugs5.

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