Bioremediation of Wastewater from Kaduna Refinery and Petrochemical Company (KPRC) Using Immobilised Burkholderia Cepacia and Corynebacterium Kutscheri

Description

– Bioremediation of Wastewater from Kaduna Refinery and Petrochemical Company (KPRC) Using Immobilised Burkholderia Cepacia and Corynebacterium Kutscheri –

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Abstract

Refinery sludge and raw wastewater samples were collected from Kaduna Refining and Petrochemical Company, Kaduna, Nigeria were collected and analyzed by selective enrichment technique.This resulted in the isolation of two bacterial species, Burkholderia cepacia and Corynebacterium kutscheri.

Crude oil degradation was performed by the pure and mixed culture of the isolates incubated in the shake flask culture.

Dehydrogenase activitie (DHA), Fluorescein diacetate (FDA) Protein Estimation, pH, Emulsification index and Optical density (OD₆₀₀) were used were used to evaluate degradation processes while CO₂ production and GC-MS were used to analyze mineralization potential.

Growth, CO₂ production, FDA and protein reached  maximum after 96h-192h of incubation while, DHA followed the same trend up to 162hours and declined.

Introduction

The Kaduna Refining and petrochemical company (KPRC) was designed to process two types of crude oils: The imported heavy crude and Nigerian light crude.

It supplies the domestic market in the Nothernwest and Northeast regions of the country (Bureau of Public Enterprise, 2003-2007).

The high demand for petroleum and associated products during the last ten decades has made petroleum spills inevitable consequences of oil production and refining.

Despite fluctuations in its prices, oil will remain a major source of energy in the next several decades because a reliable alternative has not yet been found (Adieze et al., 2003).

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