Comparative Evaluation of Parasitological And Serological Methods For The Detection Of Natural Ehrlichia Canis Infection In Dogs In Zaria Metropolis, Kaduna State, Nigeria

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– Comparative Evaluation of Parasitological And Serological Methods For The Detection Of Natural Ehrlichia Canis Infection In Dogs In Zaria Metropolis, Kaduna State, Nigeria –

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Abstract

Canine ehrlichiosis caused by Ehrlichia canis is an important and potentially fatal disease ofdogs. It has been reported worldwide and is transmitted by the dog brown tickRhipicephalussanguineus.

Detection of E. canis infection can be done by direct identification ofinclusion bodies or morulae of E.

canis in leucocytes from smears made from blood or frombuffy coat and lymphnode aspiration by immunofluorescent antibody test (IFAT) and DotELISA.

Molecular techniques such asPolymerase chain reaction (PCR) are also used asdiagnostic methods.

Introduction

Dogs are susceptible to a number of Ehrlichial diseases currently recognized to be caused byEhrlichia canis, E. chaffeensis and E. ewingii (Straube, 2010).

Among them canine ehrlichiosis(CE) caused by E. canis is an important and potentially fatal disease of dogs.

CE is an infectiousdisease caused by the gram negative bacterium E. canis (Ristic and Huxsoll, 1984) transmittedby the ubiquitous dog brown tick Rhipicephalus sanguineus (Lewis et al., 1977).

E. canisinfection has been reported as a worldwide canine infectious disease (Ristic and Holland, 1993)being more common in tropical and subtropical regions (Neer and Harrus, 2006).

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