Comparative Toxicity of Crude Oil and Kerosene on the Growth of Nitrifying Bacteria Isolated from Soil

Description

Download Comparative Toxicity of Crude Oil and Kerosene on the Growth of Nitrifying Bacteria Isolated from Soil. Microbiology students who are writing their projects can get this material to aid their research work.

Abstract

Crude oil and kerosene have useful applications in the society and are, therefore, used as sources of income and energy.

However, the spills arising from crude oil and kerosene in our environment are becoming a visible problem in our ecosystem. Crude oil and kerosene pollute our farmlands and other arable lands and may negatively affect the growth and activities of nitrifying bacteria responsible for nitrification process, leading to loss of soil fertility.

Considering the important roles played by nitrifying bacteria in soil fertility in our ecosystem, the assessment of the response of the nitrifying bacteria to pollution stress exerted by crude oil and kerosene on them was investigated.

Two genera of nitrifying bacteria were isolated from the soil and were identified as Nitrosomonas sp and Nitrobacter sp based on their morphological, biochemical and physiological characteristics.

Nitrosomonas sp was isolated using Winogradsky medium for nitrification phase I while Nitrobacter sp was isolated using Winogradsky medium for nitrification phase II.

Introduction

Crude oil and kerosene have useful applications in our society and are, therefore, used as source of income and energy.  However, the spill arising from crude oil and kerosene dumped into our environment is becoming a visible problem in our ecosystems ( Njoku et al., 2009).

The environment is increasingly exposed to changes resulting from both crude oil and refined petroleum products contamination. These changes could be drastic and as such affect the ecosystem substantially (Ikhajiagbe and Anoliefo, 2010).

Crude oil and its refined products can be released into the environment from a number of sources, which can pollute both land and water (Jane, 2010). Refined petroleum products result when crude oil is split into fractions such as petrol, kerosene, diesel oil, engine oil and many other petroleum products by a process called fractional distillation.

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