Production and Optimization of Glucoamylases from Plants and Aspergillusniger for Starch Hydrolysis in a Batch Bioreactor

Description

– Production and Optimization of Glucoamylases from Plants and Aspergillusniger for Starch Hydrolysis in a Batch Bioreactor –

Download Production and Optimization of Glucoamylases from Plants and Aspergillusniger for Starch Hydrolysis in a Batch Bioreactor. Biochemistry students who are writing their projects can get this material to aid their research work.

Abstract

Glucoamylases were produced from both plants and microorganisms and were optimized for starch hydrolysis in batch bioreactor. Amylase activity was monitored in germinating guinea corn seeds for seven days. Highest amylase activity was observed on days 3 and 7.

A study of the amylopectin content of millet, guinea corn, cassava, corn and tigernut starch showed that tiger nut had the highest amylopectin content while cassava starch had the lowest.

Moist amylopectin frommillet, guinea corn, cassava, corn and tiger nut starch were exposed on the shelf to triger microbial growth. Luxurial growths were noticed on amylopectin from guinea corn, tigernut and cassava starch.

Pure isolates were obtained by subculturing and identified as Aspergillus niger. A 14-day fermentation study to determine the optimal production time using the organism and amylopectin from guinea corn, tigernut and cassava starch was carried out.

Introduction

The huge demand for starch in industries for the production of high glucose syrups and ethanol has led to stiff competition with dietary starch. There is the need to discover other sources of starch for industrial purposes in other to spare dietary starch.

This is complicated by the problems of completely hydrolysing starch due to the difficulty of obtaining the appropriate enzymes to hydrolyse its multiple branching especially theα- 1, 6 glucosidic bonds.

The study of plant and microbial glucoamylases is important from both basic and applied perspectives. Amylase is a major enzyme in the industry (Souzaand Magalhaes, 2010).

It hydrolyses the starch molecules into glucose units (Raimi et al., 2012). Cereal grains synthesize multiple forms of α-amylase during germination to supply soluble carbohydrates for the developing seedling.

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