The Use Of Kerosene/Olive Oil proportioned Mixtures As Alternative Clearing Agent In Tissue Processing
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Description
– The Use Of Kerosene/Olive Oil proportioned Mixtures As Alternative Clearing Agent In Tissue Processing –
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Abstract
This study was aimed at investigating the potentials of different proportioned mixtures of kerosene and olive oil as alternative clearing agents in place of xylene during tissue processing.
It was carried out in the histopathology laboratory of the department of Medical Laboratory Science, Imo State University, Owerri between February and March (2017).
Sample of tissues were harvested from a presumably healthy wistar rat. These tissues were fixed and grouped based on clearant to be used. Following all basic tissue processing protocols, tissues were processed manually with only alterations of the clearing process were necessary.
Introduction
Histology deals with the study of the microscopic anatomy of tissues. For these tissues to be studied microscopically, it has to be processed by procedures known as histological techniques.
Tissue processing can be performed manually (hand processing), but where multiple specimens have to be dealt with it is more convenient and much more efficient to use an automated tissue processing machine (a “tissue processor”).
Most Histological techniques have been developed to reserve the structural integrity of the specimens so that it can be viewed microscopically (Schwann, 2008).
Tissue processing in histology is a physical process that involves chemical solutions reacting with biological specimens. Specimens from biopsies and autopsies require processing.
These processes require different procedures which include: (a) Fixation- The purpose of fixation is to preserve tissues permanently in as life-like a state as possible.
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